Studies focused on a wide spectrum of taxa, including mammals, birds and herptiles, although the majority of studies focused on fish, and typical variables measured included blood glucose, lactate and pH. We located 79 individual studies that focused on non-domesticated vertebrates, including validation and application of POC tools. Here, we review the current uses of POC devices and their applicability to basic and applied studies on a variety of non-domesticated species. The use of point-of-care (POC) devices to measure various secondary blood physiological parameters, such as metabolites, blood gases and ions, has become increasingly popular recently, due to immediate results and their portability, which allows the freedom to study organisms in the wild. In either situation, large, laboratory-bound equipment and traditional assays and analytical protocols are required. Traditionally, studies that rely on blood physiology have focused on captive animals or, in studies conducted in remote settings, have required the preservation and transport of samples for later analysis. Small, often non-lethal samples enable the assessment and monitoring of the physiological state and health of the individual. Non-human vertebrate blood is commonly collected and assayed for a variety of applications, including veterinary diagnostics and physiological research.
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